AGL1 Agrobacterium Strain
- Catalog Number: STR3006
- Packing Size: 100ul
- Storage: Store at -80 ℃
Strain Specifications & Genomic Information
| Type |
Agrobacterium tumefaciens |
| Genotype |
C58 RecA (rif R/carbR) Ti pTiBo542DT-DNA (strepR) Succinamopine |
| Resistance |
Rif (20ug/ml) / Car(50 μg/ml) |
| Culture Medium |
YEB |
| Strain Category |
Agrobacterium tumefaciens |
| Culture Conditions |
28 centigrade, aerobic, YEB |
| Transformation of Plasmids |
Electric conversion and transformation |
| Preservation Method |
30% glycerin, -80 ℃ |
| Basic Application |
Transgene |
| Documentation |
Open Protocol Sheet (PDF) |
Strain Description
AGL1 Agrobacterium Strain was C58, RecA background, containing screening label, rifampicin resistant gene RIF and carbenicillin resistance gene carb in nuclear genes, in order to facilitate the transformation of the operation, the strains carrying Ti plasmid pTiBo542DT-DNA amber base without its transport function, the plasmid containing vir gene (VIR T-DNA gene was inserted into the plant genome required the T-DNA element, pTiBo542DT-DNA plasmid transfer function is damaged, but can help the binary vector T-DNA to successfully transfer).
The pTiBo542DT-DNA Ti plasmid contains a screening tag: strep, giving AGL1 streptomycin resistance, which is suitable for transgenic operation of plants such as rice, Arabidopsis and poplar.
AGL1 strain was cultured on LB or YEB at 28 centigrade aerobic culture, and 30% glycerol could be used to preserve the bacteria.
Reference
Publication for STR3006 AGL1 Agrobacterium Strain:
- Article: CRISPR-Cas Methods
- Authors: M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla
- URI: doi.org/10.1007/978-1-0716-0616-2
Caution & Safety Information
- This product is FOR RESEARCH USE ONLY!
- This product is glycerol packed, Please kindly culture it as soon as possible then harvest and store at -80℃.
- Shipping temperature is 2-8℃.
