Bovine ACCS (1- aminocyclopropane- 1- carboxylate synthase- like protein 1) ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Bovine ACCS ELISA KIT ,Bovine 1 aminocyclopropane 1 carboxylate synthase ELISA KIT ,Bovine ACS ELISA KIT ,Bovine PHACS ELISA KIT ,Bovine 1-aminocyclopropane-1-carboxylate synthase-like protein (non-functional) isoform 1 ELISA KIT ,Bovine 1-aminocyclopropane-1-carboxylate synthase-like protein (non-functional) isoform 2 ELISA KIT ,Bovine 1-aminocyclopropane-1-carboxylate synthase-like protein (non-functional) isoform 4 ELISA KIT ,Bovine ACC synthase-like protein 1 ELISA KIT ,Bovine 1-aminocyclopropane-1-carboxylate synthase homolog ELISA KIT

Intended use

Bovine ACCS ELISA kit allows for the in vitro quantitative determination of Bovine 1- aminocyclopropane- 1- carboxylate synthase- like protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Bovine ACCS ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACCS, During the reaction, ACCS in the sample or standard competes with a fixed amount of biotin-labeled ACCS for sites on a pre-coated Monoclonal antibody specific to ACCS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACCS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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