Canine Phosphodiesterase 5A cGMP Specific, PDE5A ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
cGMP-specific 3',5'-cyclic phosphodiesterase,PDE5A,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE, PDE5A,CGB-PDE; CN5A; PDE5; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; phosphodiesterase isozyme 5; phosphodiesterase 5A cGMP-specific
Search name
Canine PDE5A ELISA KIT ,Canine cGMP-specific 3' ELISA KIT ,Canine 5'-cyclic phosphodiesterase ELISA KIT , Canine cGMP-binding c ELISA KIT , Canine GMP-specific phosphodiesterase ELISA KIT ,Canine CGB-PDE ELISA KIT ,Canine CGB-PDE ELISA KIT ,Canine CN5A ELISA KIT ,Canine PDE5 ELISA KIT ,Canine cGMP-binding cGMP-specific 3' ELISA KIT ,Canine 5'-cyclic nucleotide phosphodiesterase ELISA KIT ,Canine cGMP-specific 3' ELISA KIT ,Canine 5'-cyclic phosphodiesterase ELISA KIT ,Canine cGMP-specific phosphodiesterase PDE5A2 ELISA KIT ,Canine cGMP-specific phosphodiesterase type 5A ELISA KIT ,Canine phosphodiesterase isozyme 5 ELISA KIT ,Canine phosphodiesterase 5A cGMP-specific ELISA KIT
Intended use
Canine PDE5A ELISA KIT allows for the in vitro quantitative determination of Canine cgmp-specific 3',5'-cyclic phosphodiesterase,PDE5A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Canine PDE5A ELISA KIT has been pre-coated with an antibody specific to PDE5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDE5A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDE5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDE5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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