EHA105 Agrobacterium Strain
Product Overview: The EHA105 Agrobacterium tumefaciens strain is a highly efficient tool for plant genetic transformation, derived from the C58 background. It carries the pEHA105 helper plasmid, which facilitates T-DNA transfer while maintaining high stability. With rifampicin resistance and optimal growth at 28°C on YEB or LB media, EHA105 is widely used for transient and stable expression studies in diverse plant species. Its robust transformation capabilities and binary vector compatibility make it an essential resource for advanced functional genomics and agricultural biotechnology research.
Pricing & Availability
| Catalog No. |
Packing |
Storage |
| STR3004 |
100ul |
-80℃ |
Technical Specifications
| Genotype | C58 (rif R), Ti pEHA105 (pTiBo542DT-DNA) Succinamopine |
|---|
| Resistance | Rifampicin (Rif) |
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| Culture Medium | YEB |
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| Optimal Temperature | 28℃, aerobic |
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| Transformation | Chemical/Electronic conversion |
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| Preservation | 30% glycerin, -80℃ |
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Description
The EHA105 strain, based on the EHA101 strain and C58 background, features rifampicin resistance (RIF) in nuclear genes for easy screening. To facilitate transformation, it carries the Ti plasmid pEHA105 (pTiBo542DT-DNA), which lacks its own transport function but contains the essential VIR genes required for T-DNA insertion into the plant genome. The plasmid's T-DNA transfer function is natively destroyed, but it effectively assists the transfer of binary vector T-DNA. EHA105 can be cultured on YEB or LB media at 28°C under aerobic conditions.
Operating Instructions
- This product contains a portion of glycerin bacteria. When using, you can dip a small amount for plate streaking or liquid culture without complete melting. It can also be used after complete thawing, but please note that activity gradually declines with repeated freeze-thaw cycles.
- In order to ensure the purity of bacteria and avoid contamination, it is recommended to first draw the plate, and then select a single monoclone colony for subsequent experiments.
Antibiotics & Media Preparation
Antibiotics Preparation
| Antibiotics | Method | Stock Solution | Fluid Conc. |
|---|
| Carb | Dissolved in DDW, filter sterilized (0.22μm) | 50mg/ml | 50ug/ml |
| Kan | Dissolved in DDW, filter sterilized (0.22μm) | 50mg/ml | 50ug/ml |
| Strep | Dissolved in DDW, filter sterilized (0.22μm) | 10mg/ml | 50ug/ml |
| Rif | Dissolved in DMSO, filter sterilized (0.22μm) | 10mg/ml | 20ug/ml |
| Gent | Dissolved in DDW, filter sterilized (0.22μm) | 20mg/ml | 40ug/ml |
LB and YEB Media Composition
| Component | LB (Liquid) | LB (Solid) | Component | YEB (Liquid) | YEB (Solid) |
|---|
| Tryptone | 10g | 10g | Tryptone | 5g | 5g |
| Yeast Extract | 5g | 5g | Yeast Extract | 1g | 1g |
| NaCl | 10g | 10g | Beef Extract | 5g | 5g |
| NaOH | PH to 7 | PH to 7 | Sucrose | 5g | 5g |
| Agar | - | 15g | MgSO4*7H2O | 0.49g | 0.49g |
| - | - | - | NaOH/Agar | PH to 7 | PH to 7 / 15g |
Caution
- 1. This product is FOR RESEARCH USE ONLY!
- 2. Please kindly culture it as soon as possible then harvest and store at -80℃.
Frequently Asked Questions (FAQ)
Q: What makes EHA105 superior for plant transformation?
A: EHA105 is derived from the EHA101/C58 background and contains a modified Ti plasmid (pTiBo542DT-DNA) that is optimized for binary vector systems, making it highly effective for T-DNA transfer in recalcitrant plant species.
Q: How should I manage freeze-thaw cycles?
A: Bacterial viability decreases with each cycle. It is highly recommended to create secondary glycerol stocks or streak plates immediately upon receiving the product to minimize the number of times the primary stock is thawed.
Q: Why is rifampicin used for EHA105 selection?
A: The EHA105 strain naturally carries a rifampicin resistance gene in its nuclear genome, which serves as a selectable marker to confirm the identity of the strain and prevent contamination from other non-Agrobacterium species.
Citations & References
- [1] Tomasz Kowalczyk, et al. (2018). High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes. AMB Express. doi: 10.1186/s13568-018-0656-6.
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