GV2260 Agrobacterium Strain
Product Overview: The GV2260 Agrobacterium tumefaciens strain is a premier tool for plant genetic transformation, widely used in model systems like Arabidopsis and various crops. Featuring a C58 chromosomal background and the octopine-type Ti plasmid pGV2260, it ensures stable T-DNA integration. With verified antibiotic resistance (Rif/Carb) and optimized culture protocols, this strain offers high-efficiency intergeneric gene transfer and excellent compatibility with binary vectors. It is a robust, essential resource for advanced molecular plant biology, functional genomics, and transgenic research workflows.
Pricing & Availability
| Catalog No. |
Product Name |
Packing |
Storage |
| STR3008 |
GV2260 Agrobacterium Strain |
100ul |
-80°C |
Technical Specifications & Genotype
| Strain Category | Agrobacterium tumefaciens |
|---|
| Chromosomal Background | C58 |
|---|
| Genotype | C58, RifR, CarbR, Ti plasmid pTiB6S3DT-DNA (Octopine) |
|---|
| Antibiotic Resistance | Rif (20ug/ml), Carb (50ug/ml) |
|---|
| Culture Conditions | 30°C, aerobic, LB |
|---|
| Transformation | Electric conversion |
|---|
Genomic Toxin-Antitoxin (TA) Systems
| TA ID |
MGEs |
Organism (Replicon) |
Accession |
Toxin |
Antitoxin |
Family |
| in_silico TA185597 |
- |
Agrobacterium fabrum GV2260 (chr) |
NZ_CP067027 |
EML4058_RS01755 (pasB) |
EML4058_RS01760 (pasA) |
pasABC/RelE-HTH |
| in_silico TA185598 |
- |
Agrobacterium fabrum GV2260 (chr) |
NZ_CP067027 |
EML4058_RS03045 (relE) |
EML4058_RS03050 (dinJ) |
relE-dinJ |
| in_silico TA185599 |
- |
Agrobacterium fabrum GV2260 (chr) |
NZ_CP067027 |
EML4058_RS03080 (mazF) |
EML4058_RS03075 (mazE) |
mazEF/MazF-MazE |
| in_silico TA185600 |
- |
Agrobacterium fabrum GV2260 (chr) |
NZ_CP067027 |
EML4058_RS08290 (yoeB) |
EML4058_RS08295 (yefM) |
yefM-yoeB |
Frequently Asked Questions (FAQ)
Q: What is the significance of the listed TA systems in GV2260?
A: These toxin-antitoxin (TA) systems are stress-response modules inherent to the strain's chromosome. Understanding these loci (like MazEF and RelBE) is critical for researchers analyzing stability, survival under stress, and genomic integrity during large-scale transgenic cultivations.
Q: How should I manage antibiotic selection?
A: Use Rifampicin (20ug/ml) and Carbenicillin (50ug/ml) for maintenance. Always ensure your plates contain the correct concentrations to prevent growth of non-transformed contaminants.
Citations & References
(Editor's Note: Please update the citations below to reflect your specific laboratory literature source.)
- [1] Genomic Reference: NZ_CP067027.
- [2] Lab-specific guidelines on Agrobacterium-mediated plant transformation protocols.
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