Human Nitric oxide synthase, brain, NOS1 ELISA KIT

Product Name:Human Nitric oxide synthase, brain, NOS1 ELISA KIT
Packing:96T

Catalog No.:ELI-02230h

Gene Name:NOS1

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Nitric oxide synthase(brain(NOS1

Alternative Name:NOS1,Nitric oxide synthase(brain(NOS1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Nitric oxide synthase, brain, NOS1 ELISA KIT allows for the in vitro quantitative determination of Nitric oxide synthase(brain(NOS1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Nitric oxide synthase, brain, NOS1 ELISA KIT has been pre-coated with an Nitric oxide synthase(brain(NOS1 antibody specific to Nitric oxide synthase(brain(NOS1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nitric oxide synthase(brain(NOS1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nitric oxide synthase(brain(NOS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Nitric oxide synthase(brain(NOS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!