Human PRDX1 ELISA Kit

Product Name:Human PRDX1 ELISA Kit
Packing:96T

Catalog No.:ELA-E2222h

Gene Name:PRDX1

Detect Range:15.6-1000 pg/mL

Sensitivity:10pg/mL

Target Protein Name:Human Peroxiredoxin-1

Alternative Name:Peroxiredoxin-1, Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PRDX1 ELISA Kit allows for the in vitro quantitative determination of Human Peroxiredoxin-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PRDX1 ELISA Kit has been pre-coated with an PRDX1 antibody specific to Human Peroxiredoxin-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Peroxiredoxin-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Peroxiredoxin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Peroxiredoxin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!