Human Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform, PIK3CA ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PIK3CA,p110,serine/threonine protein kinase PIK3CA; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; p110-alpha; PI3-kinase p110 subunit alpha; PI3K-alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; phosphoinositide-3-kinase, catalytic, alpha polypeptide; ptdIns-3-kinase subunit p110-alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
Search name
Human PIK3CA ELISA KIT ,Human p110 ELISA KIT ,Human serine/threonine protein kinase PIK3CA ELISA KIT ,Human CLOVE ELISA KIT ,Human CWS5 ELISA KIT ,Human MCAP ELISA KIT ,Human MCM ELISA KIT ,Human MCMTC ELISA KIT ,Human PI3K ELISA KIT ,Human p110-alpha ELISA KIT ,Human PI3-kinase p110 subunit alpha ELISA KIT ,Human PI3K-alpha ELISA KIT ,Human phosphatidylinositol 3-kinase catalytic110-KD alpha ELISA KIT ,Human phosphatidylinositol 3-kinase catalytic alpha polypeptide ELISA KIT, Human phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA KIT, Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA KIT, Human phosphoinositide-3-kinase catalytic alpha polypeptide ELISA KIT, Human ptdIns-3-kinase subunit p110-alpha ELISA KIT, Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha ELISA KIT
Intended use
Human PIK3CA ELISA KIT allows for the in vitro quantitative determination of Human Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform, PIK3CA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human PIK3CA ELISA KIT has been pre-coated with an antibody specific to Human Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Phosphatidylinositol- 4,5- bisphosphate 3-kinase catalytic subunit alpha isoform,PIK3CA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human PIK3CA ELISA KIT Range 0.15-10ng/ml
PIK3CA Related Products
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