LBA9402 Agrobacterium Strain
Product Overview: The LBA9402 *Agrobacterium rhizogenes* strain is a powerful tool for plant genetic transformation, specifically engineered for hairy root induction. Featuring Rifampicin resistance, this strain is vital for research in molecular biology, metabolic engineering, and plant functional genomics. Compatible with both chemical and electrochemical transformation protocols, LBA9402 ensures high efficiency and stability. It is an essential asset for scientists focusing on stable transgenic plant development, providing reliable results for diverse botanical applications and gene expression studies.
Pricing & Availability
| Catalog No. |
Packing |
Storage |
| STR3002 |
100ul |
-80℃ |
Technical Specifications
| Type | Agrobacterium rhizogenes |
|---|
| Classification | Agrobacterium tumefaciens |
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| Resistance | Rif |
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| Culture Medium | LB |
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| Culture Conditions | 30℃, aerobic, LB |
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| Transformation | Electrochemical and chemical |
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| Preservation | 30% glycerol, -80℃ |
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Description
The LBA9402 Agrobacterium strain is specifically optimized for genetic modification research. It exhibits the characteristics of Agrobacterium rhizogenes, making it highly effective for gene transfer experiments in plant science. The strain is maintained at -80℃ to ensure viability and genomic stability.
Operating Instructions
1. This product contains a portion of glycerin bacteria. When using this glycerin bacteria, you can dip a small amount of coated plate or liquid culture on the surface of glycerin bacteria without complete melting. It can also be used after complete thawing, but with the increase of freeze-thaw times, the activity of bacteria will gradually decline.
2. In order to ensure the purity of bacteria and avoid other bacterial contamination, we should first draw the plate, and then select the monoclone colony for subsequent operation.
Caution
- 1. This product is FOR RESEARCH USE ONLY!
- 2. Please kindly culture it as soon as possible then harvest and store at -80℃.
Frequently Asked Questions (FAQ)
Q: What is the primary application of LBA9402?
A: LBA9402 is an *Agrobacterium rhizogenes* strain primarily used for plant genetic modification, particularly effective for inducing hairy root systems and facilitating stable gene integration in various plant species.
Q: Why should I perform plate streaking before use?
A: Plate streaking is a critical step to ensure the purity of the culture. It allows you to isolate monoclone colonies, thereby avoiding bacterial contamination and ensuring the reliability of your genetic transformation experiments.
Q: Does repeated freeze-thaw affect the strain?
A: Yes. While the bacteria can be used after thawing, excessive freeze-thaw cycles significantly decrease the viability and biological activity of the strain. We recommend aliquotting or using the stock efficiently to minimize cycles.
Citations & References
(Editor's Note: Please update the citations below to reflect relevant literature.)
- [1] (Example): Tzfira, T., & Citovsky, V. (2006). Agrobacterium-mediated genetic transformation of plants: biology and biotechnology.
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