Mouse Igh-VJ558 3 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
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Intended use
Mouse Igh-VJ558 ELISA kit allows for the in vitro quantitative determination of Mouse Igh-VJ558 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse Igh-VJ558 3 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Igh-VJ558 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Igh-VJ558, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Igh-VJ558 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Igh-VJ558 3 ELISA kit Range 0.15-10ng/ml
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