Rat CCK ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat CCK ELISA KIT ,Rat Cholecystokinin ELISA KIT ,Rat prepro-cholecystokinin ELISA KIT ,Rat cholecystokinin triacontatriapeptide ELISA KIT
Intended use
Rat CCK ELISA kit allows for the in vitro quantitative determination of Rat CCK concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Rat CCK ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat CCK ELISA kit Range 7.8-500pg/ml
CCK Related Products
> CCK ELISA KITS
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