Rat Lysophosphatidic acid receptor 1, LPAR1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
LPAR1,lysophosphatidic acid receptor 1; lysophosphatidic acid receptor Edg-2; EDG2; GPR26; Gpcr26; LPA1; Mrec1.3; VZG1; edg-2; rec.1.3; vzg-1; LPA receptor 1; LPA-1; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2; ventricular zone gene 1,Lysophosphatidic acid receptor 1,LPA receptor 1,LPA-1,Lysophosphatidic acid receptor Edg-2,
Search name
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Intended use
Rat LPAR1 ELISA KIT allows for the in vitro quantitative determination of Rat Lysophosphatidic acid receptor 1, LPA receptor 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat LPAR1 ELISA KIT has been pre-coated with an antibody specific to LPA receptor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LPA receptor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain LPA receptor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LPA receptor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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