Rat Sarcolipin, SLN ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SLN,sarcolipin
Search name
Rat SLN ELISA KIT ,Rat sarcolipin ELISA KIT
Intended use
Rat sarcolipin ELISA KIT allows for the in vitro quantitative determination of Rat Sarcolipin, SLN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat sarcolipin ELISA KIT has been pre-coated with an antibody specific to Rat Sarcolipin, SLN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Sarcolipin, SLN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Sarcolipin, SLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Sarcolipin, SLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat sarcolipin ELISA KIT Range 0.15-10ng/ml
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