Rat Septin- 9, SEPT9 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SEPT9,AF17q25; MSF; MSF1; NAPB; PNUTL4; SINT1; SeptD1; MLL septin-like fusion protein MSF-A; Ov/Br septin; ovarian/breast septin; septin D1; septin 9

Search name

Rat SEPT9 ELISA KIT ,Rat AF17q25 ELISA KIT ,Rat MSF ELISA KIT ,Rat MSF1 ELISA KIT ,Rat NAPB ELISA KIT ,Rat PNUTL4 ELISA KIT ,Rat SINT1 ELISA KIT ,Rat SeptD1 ELISA KIT ,Rat MLL septin-like fusion protein MSF-A ELISA KIT ,Rat Ov/Br septin ELISA KIT ,Rat ovarian/breast septin ELISA KIT ,Rat septin D1 ELISA KIT ,Rat septin 9 ELISA KIT

Intended use

Rat SEPT9 ELISA KIT allows for the in vitro quantitative determination of Rat Septin- 9, SEPT9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rat SEPT9 ELISA KIT has been pre-coated with an antibody specific to Rat Septin- 9, SEPT9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Septin- 9, SEPT9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Septin- 9, SEPT9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Septin- 9, SEPT9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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