Bovine Glucose- 6- Phosphate Dehydrogenase ELISA Kit
Product Name:Bovine Glucose- 6- Phosphate Dehydrogenase ELISA Kit
Packing:96T
Catalog No.:ELA-E0716b
Gene Name:Bovine Glucose-6-Phosphate Dehydrogenase
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Glucose-6-Phosphate Dehydrogenase
Alternative Name:G6PD,Bovine Glucose-6-Phosphate Dehydrogenase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Glucose- 6- Phosphate Dehydrogenase ELISA Kit allows for the in vitro quantitative determination of Bovine Glucose-6-Phosphate Dehydrogenase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Glucose- 6- Phosphate Dehydrogenase ELISA Kit has been pre-coated with an Bovine Glucose-6-Phosphate Dehydrogenase antibody specific to Bovine Glucose-6-Phosphate Dehydrogenase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Glucose-6-Phosphate Dehydrogenase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Glucose-6-Phosphate Dehydrogenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Glucose-6-Phosphate Dehydrogenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!