USP10 antibody

Product Name USP10 antibody

Catalog No PAab10128

Packing 100ul

Form liquid

Alternative Name Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10

Purification Immunogen affinity purified

Purity 95% as determined by SDS-PAGE

Host Rabbit

Isotype IgG

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimulation or upon DNA damage(PubMed:25861989).

Immunogen ubiquitin specific peptidase 10

Specificity Human, Mouse

Tested Application ELISA, IHC, IF

Recommended dilution IHC: 1:50-1:200; IF: 1:20-1:100

IHC use Immunohistochemistry of paraffin-embedded mouse brain tissue slide using PAab10128 (USP10 Antibody) at dilution of 1:100.

WB use

Protein Information

Gene ID 9100

Uniprot ID Q14694

Calculated MW

Research Area Epigenetics, Metabolism