Bovine 10 kDa heat shock protein, mitochondrial, HSPE1 ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
10 kDa heat shock protein, mitochondrial(Hsp10),Early-pregnancy factor(EPF),10 kDa chaperonin,Chaperonin 10(CPN10),;
HSPE1,CPN10; EPF; GROES; HSP10; 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; chaperonin 10; early-pregnancy
factor; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10)

Search name
Bovine 10 kDa heat shock protein ELISA Kit, Bovine chaperonin 10 ELISA Kit, Bovine CPN10 ELISA Kit , Bovine HSP10 ELISA
Kit, Bovine Early-pregnancy factor ELISA Kit, Bovine EPF ELISA Kit, Bovine HSPE1 ELISA Kit, Bovine GROES ELISA Kit, 10 kDa
heat shock protein ELISA Kit, chaperonin 10 ELISA Kit, CPN10 ELISA Kit , HSP10 ELISA Kit, Early-pregnancy factor ELISA Kit,
EPF ELISA Kit, HSPE1 ELISA Kit, GROES ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine 10 kda heat shock protein, mitochondrial,Hsp10
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hsp10. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hsp10 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Hsp10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hsp10 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.