Bovine 3- ketoacyl- CoA thiolase, mitochondrial, ACAA2 ELISA KIT

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  • Model: ELI-52039b
  • 20 Units in Stock
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Bovine 3- ketoacyl- CoA thiolase, mitochondrial, ACAA2 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

ACAA2,mitochondrial 3 oxoacyl CoA thiolase,DSAEC; 3-ketoacyl-CoA thiolase, mitochondrial; acetyl-Coenzyme A acyltransferase 2; beta ketothiolase; beta-ketothiolase; mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; acetyl-CoA acyltransferase 2

 

Search name

Bovine ACAA2 ELISA KIT ,Bovine mitochondrial 3 oxoacyl CoA thiolase ELISA KIT ,Bovine DSAEC ELISA KIT ,Bovine 3-ketoacyl-CoA thiolase mitochondrial ELISA KIT ,Bovine acetyl-Coenzyme A acyltransferase 2 ELISA KIT ,Bovine beta ketothiolase ELISA KIT ,Bovine beta-ketothiolase ELISA KIT ,Bovine mitochondrial 3-oxoacyl-CoA thiolase ELISA KIT ,Bovine mitochondrial 3-oxoacyl-Coenzyme A thiolase ELISA KIT ,Bovine acetyl-CoA acyltransferase 2 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine 3- ketoacyl- CoA thiolase, mitochondrial, ACAA2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACAA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ACAA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACAA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACAA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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