Bovine 40S ribosomal protein S3a, RPS3A ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
RPS3A,FTE1; MFTL; S3A; 40S ribosomal protein S3a; fte-1; v-fos transformation effector protein 1; ribosomal protein S3A
Search name
Bovine RPS3A ELISA KIT ,Bovine FTE1 ELISA KIT ,Bovine MFTL ELISA KIT ,Bovine S3A ELISA KIT ,Bovine 40S ribosomal protein S3a ELISA KIT ,Bovine fte-1 ELISA KIT ,Bovine v-fos transformation effector protein 1 ELISA KIT ,Bovine ribosomal protein S3A ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine 40S ribosomal protein S3a, RPS3A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPS3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RPS3A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RPS3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RPS3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.