Bovine 5'-AMP-activated protein kinase subunit beta-1, AMPK ELIS

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  • Model: ELI-05469b
  • 20 Units in Stock
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Bovine 5'-AMP-activated protein kinase subunit beta-1, AMPK ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
5'-AMP-activated protein kinase subunit beta-1,AMPK subunit beta-1,AMPKb,,AMPK,HAMPKb,5'-AMP-activated protein kinase beta-1
subunit,5'-AMP-activated protein kinase subunit beta-1,AMP-activated protein kinase beta subunit,AMPK beta -1 chain,AMPK beta
1,AMPK subunit beta-1,AMPKb,protein kinase, AMP-activated, noncatalytic, beta-1,protein kinase, AMP-activated, beta 1 non-catalytic
subunit

Search name
Bovine 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit,Bovine PRKAB ELISA Kit, 5'-AMP-activated protein kinase
subunit beta-1 ELISA Kit,PRKAB ELISA Kit,ELISA Kit,5'-AMP-activated protein kinase subunit beta-1 ELISA Kit,AMPK subunit
beta-1 ELISA Kit,AMPKb ELISA Kit, ELISA Kit,AMPK ELISA Kit,HAMPKb ELISA Kit,5'-AMP-activated protein kinase beta-1
subunit ELISA Kit,5'-AMP-activated protein kinase subunit beta-1 ELISA Kit,AMP-activated protein kinase beta subunit ELISA
Kit,AMPK beta -1 chain ELISA Kit,AMPK beta 1 ELISA Kit,AMPK subunit beta-1 ELISA Kit,AMPKb ELISA Kit,protein kinase ELISA
Kit, AMP-activated ELISA Kit, noncatalytic ELISA Kit, beta-1 ELISA Kit,protein kinase ELISA Kit, AMP-activated ELISA Kit, beta 1
non-catalytic subunit ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine 5'-amp-activated protein kinase subunit beta-1,AMPK
subunit beta-1 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMPK subunit beta-1. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMPK subunit
beta-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain AMPK subunit beta-1, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMPK subunit beta-1 in the samples
is then determined by comparing the O.D. of the samples to the standard curve.

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