Bovine Acidic mammalian chitinase, Chia ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Acidic mammalian chitinase,YNL, CHIA,AMCASE; CHIT2; TSA1902; acidic mammalian chitinase; lung-specific protein TSA1902; chitinase, acidic

Search name

Bovine Acidic mammalian chitinase ELISA KIT ,Bovine YNL ELISA KIT ,Bovine  CHIA ELISA KIT ,Bovine AMCASE ELISA KIT ,Bovine CHIT2 ELISA KIT ,Bovine TSA1902 ELISA KIT ,Bovine acidic mammalian chitinase ELISA KIT ,Bovine lung-specific protein TSA1902 ELISA KIT ,Bovine chitinase acidic ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine acidic mammalian chitinase,AMCase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMCase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMCase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMCase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMCase in the samples is then determined by comparing the O.D. of the samples to the standard curve.