Bovine Adenine phosphoribosyltransferase, APRT ELISA KIT

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  • Model: ELI-24357b
  • 20 Units in Stock
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Bovine Adenine phosphoribosyltransferase, APRT ELISA KIT

Product Name:Bovine Adenine phosphoribosyltransferase, APRT ELISA KIT
Packing:96T

Catalog No.:ELI-24357b

Gene Name:Bovine Adenine phosphoribosyltransferase, APRT

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine Adenine phosphoribosyltransferase, APRT

Alternative Name:APRT,Bovine Adenine phosphoribosyltransferase, APRT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Adenine phosphoribosyltransferase, APRT ELISA KIT allows for the in vitro quantitative determination of Bovine Adenine phosphoribosyltransferase, APRT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Adenine phosphoribosyltransferase, APRT ELISA KIT has been pre-coated with an Bovine Adenine phosphoribosyltransferase, APRT antibody specific to Bovine Adenine phosphoribosyltransferase, APRT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Adenine phosphoribosyltransferase, APRT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Adenine phosphoribosyltransferase, APRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Adenine phosphoribosyltransferase, APRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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