Bovine Aspartyl/asparaginyl beta- hydroxylase, ASPH ELISA KIT

Product Name:Bovine Aspartyl/asparaginyl beta- hydroxylase, ASPH ELISA KIT
Packing:96T

Catalog No.:ELI-11993b

Gene Name:Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH

Alternative Name:ASPH,Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Aspartyl/asparaginyl beta- hydroxylase, ASPH ELISA KIT allows for the in vitro quantitative determination of Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Aspartyl/asparaginyl beta- hydroxylase, ASPH ELISA KIT has been pre-coated with an Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH antibody specific to Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Aspartyl(asparaginyl beta-hydroxylase, ASPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!