Bovine ATP synthase subunit d, mitochondrial, ATP5H ELISA KIT

Product Name:Bovine ATP synthase subunit d, mitochondrial, ATP5H ELISA KIT
Packing:96T

Catalog No.:ELI-34317b

Gene Name:Bovine ATP synthase subunit d, mitochondrial, ATP5H

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Bovine ATP synthase subunit d, mitochondrial, ATP5H

Alternative Name:ATP5H,Bovine ATP synthase subunit d, mitochondrial, ATP5H
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine ATP synthase subunit d, mitochondrial, ATP5H ELISA KIT allows for the in vitro quantitative determination of Bovine ATP synthase subunit d, mitochondrial, ATP5H concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine ATP synthase subunit d, mitochondrial, ATP5H ELISA KIT has been pre-coated with an Bovine ATP synthase subunit d, mitochondrial, ATP5H antibody specific to Bovine ATP synthase subunit d, mitochondrial, ATP5H .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine ATP synthase subunit d, mitochondrial, ATP5H and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine ATP synthase subunit d, mitochondrial, ATP5H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine ATP synthase subunit d, mitochondrial, ATP5H in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!