Bovine Bone morphogenetic protein 3B, BMP3B ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

GDF10,BMP-3b; BMP3B; BIP; GDF-10; bone morphogenetic protein 3B; bone-inducing protein; growth differentiation factor 10, Growth/differentiation factor 10

Search name

Bovine GDF10 ELISA KIT ,Bovine BMP-3b ELISA KIT ,Bovine BMP3B ELISA KIT ,Bovine BIP ELISA KIT ,Bovine GDF-10 ELISA KIT ,Bovine bone morphogenetic protein 3B ELISA KIT ,Bovine bone-inducing protein ELISA KIT ,Bovine growth differentiation factor 10 ELISA KIT ,Bovine Growth/differentiation factor 10 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine bone morphogenetic protein 3b,BMP-3B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP-3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BMP-3B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BMP-3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BMP-3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.