Bovine Bone sialoprotein 2, IBSP ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Bone sialoprotein 2,Integrin-binding sialoprotein,Bone sialoprotein II,BSP II,Cell-binding sialoprotein, IBSP,BNSP; BSP; BSP-II; SP-II; BSP II; bone sialoprotein 2; bone sialoprotein II; cell-binding sialoprotein; integrin-binding sialoprotein

Search name

Bovine Bone sialoprotein 2 ELISA KIT ,Bovine Integrin-binding sialoprotein ELISA KIT ,Bovine Bone sialoprotein II ELISA KIT ,

Bovine BSP II ELISA KIT ,Bovine Cell-binding sialoprotein ELISA KIT ,Bovine  IBSP ELISA KIT ,Bovine BNSP ELISA KIT ,

Bovine BSP ELISA KIT ,Bovine BSP-II ELISA KIT ,Bovine SP-II ELISA KIT ,Bovine BSP II ELISA KIT ,Bovine bone sialoprotein 2 ELISA KIT ,Bovine bone sialoprotein II ELISA KIT ,Bovine cell-binding sialoprotein ELISA KIT ,Bovine integrin-binding sialoprotein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Bone sialoprotein 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone sialoprotein 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bone sialoprotein 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bone sialoprotein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bone sialoprotein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.