Bovine Calcitonin gene- related peptide 1, CGRP ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Calcitonin gene-related peptide 1,Alpha-type CGRP,Calcitonin gene-related peptide I(CGRP-I), CALC1; CGRP; CGRP-I; CGRP1;
alpha-type CGRP; calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin;
calcitonin-related polypeptide alpha

Search name
Bovine Calcitonin gene-related peptide 1 ELISA Kit, Bovine CGRP ELISA Kit, Bovine Alpha-type CGRP ELISA Kit, Bovine
Calcitonin gene-related peptide I ELISA Kit, Bovine CGRP-I ELISA Kit, Bovine CALC1ELISA Kit, Bovine CGRP-I ELISA Kit,
Bovine CGRP1 ELISA Kit, Bovine calcitonin 1 ELISA Kit, Bovine calcitonin/calcitonin-related polypeptide alpha ELISA Kit,
Bovine katacalcin ELISA Kit, Bovine calcitonin-related polypeptide alpha ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine calcitonin gene-related peptide 1,CGRP concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CGRP. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CGRP and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain CGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CGRP in the samples is then determined by comparing
the O.D. of the samples to the standard curve.