Bovine Carboxypeptidase E, CPE ELISA KIT

Starting at: $796.00

  • Model: ELI-50277b
  • 20 Units in Stock
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Bovine Carboxypeptidase E, CPE ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

CPE,carboxypeptidase E; CPH; carboxypeptidase H; cobalt-stimulated chromaffin granule carboxypeptidase; enkephalin convertase; insulin granule-associated carboxypeptidase; prohormone-processing carboxypeptidase

 

Search name

Bovine CPE ELISA KIT ,Bovine carboxypeptidase E ELISA KIT ,Bovine CPH ELISA KIT ,Bovine carboxypeptidase H ELISA KIT ,Bovine cobalt-stimulated chromaffin granule carboxypeptidase ELISA KIT ,Bovine enkephalin convertase ELISA KIT ,Bovine insulin granule-associated carboxypeptidase ELISA KIT ,Bovine prohormone-processing carboxypeptidase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Carboxypeptidase E, CPE concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CPE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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