Bovine Centromere protein M, CENPM ELISA KIT
Product Name:Bovine Centromere protein M, CENPM ELISA KIT
Packing:96T
Catalog No.:ELI-10163b
Gene Name:Bovine Centromere protein M, CENPM
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Centromere protein M, CENPM
Alternative Name:CENPM,Bovine Centromere protein M, CENPM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Centromere protein M, CENPM ELISA KIT allows for the in vitro quantitative determination of Bovine Centromere protein M, CENPM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Centromere protein M, CENPM ELISA KIT has been pre-coated with an Bovine Centromere protein M, CENPM antibody specific to Bovine Centromere protein M, CENPM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Centromere protein M, CENPM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Centromere protein M, CENPM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Centromere protein M, CENPM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!