Bovine Cytochrome b- 245 heavy chain, CYBB ELISA KIT

Product Name:Bovine Cytochrome b- 245 heavy chain, CYBB ELISA KIT
Packing:96T

Catalog No.:ELI-25834b

Gene Name:Bovine Cytochrome b-245 heavy chain, CYBB

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine Cytochrome b-245 heavy chain, CYBB

Alternative Name:CYBB,Bovine Cytochrome b-245 heavy chain, CYBB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Cytochrome b- 245 heavy chain, CYBB ELISA KIT allows for the in vitro quantitative determination of Bovine Cytochrome b-245 heavy chain, CYBB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Cytochrome b- 245 heavy chain, CYBB ELISA KIT has been pre-coated with an Bovine Cytochrome b-245 heavy chain, CYBB antibody specific to Bovine Cytochrome b-245 heavy chain, CYBB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Cytochrome b-245 heavy chain, CYBB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Cytochrome b-245 heavy chain, CYBB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Cytochrome b-245 heavy chain, CYBB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!