Bovine Cytochrome c, CYCS ELISA KIT
Product Name:Bovine Cytochrome c, CYCS ELISA KIT
Packing:96T
Catalog No.:ELI-02143b
Gene Name:Bovine Cytochrome c, CYCS
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Cytochrome c, CYCS
Alternative Name:CYCS,Bovine Cytochrome c, CYCS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Cytochrome c, CYCS ELISA KIT allows for the in vitro quantitative determination of Bovine Cytochrome c, CYCS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Cytochrome c, CYCS ELISA KIT has been pre-coated with an Bovine Cytochrome c, CYCS antibody specific to Bovine Cytochrome c, CYCS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Cytochrome c, CYCS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Cytochrome c, CYCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Cytochrome c, CYCS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!