Bovine Cytochrome c- type heme lyase, HCCS ELISA KIT
Product Name:Bovine Cytochrome c- type heme lyase, HCCS ELISA KIT
Packing:96T
Catalog No.:ELI-33976b
Gene Name:Bovine Cytochrome c-type heme lyase, HCCS
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Cytochrome c-type heme lyase, HCCS
Alternative Name:HCCS,Bovine Cytochrome c-type heme lyase, HCCS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Cytochrome c- type heme lyase, HCCS ELISA KIT allows for the in vitro quantitative determination of Bovine Cytochrome c-type heme lyase, HCCS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Cytochrome c- type heme lyase, HCCS ELISA KIT has been pre-coated with an Bovine Cytochrome c-type heme lyase, HCCS antibody specific to Bovine Cytochrome c-type heme lyase, HCCS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Cytochrome c-type heme lyase, HCCS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Cytochrome c-type heme lyase, HCCS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Cytochrome c-type heme lyase, HCCS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!