Bovine PHGDH ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Bovine PHGDH ELISA KIT ,Bovine phosphoglycerate dehydrogenase ELISA KIT ,Bovine 3-PGDH ELISA KIT ,Bovine 3PGDH ELISA KIT ,Bovine HEL-S-113 ELISA KIT ,Bovine NLS ELISA KIT ,Bovine NLS1 ELISA KIT ,Bovine PDG ELISA KIT ,Bovine PGAD ELISA KIT ,Bovine PGD ELISA KIT ,Bovine PGDH ELISA KIT ,Bovine PHGDHD ELISA KIT ,Bovine SERA ELISA KIT ,Bovine D-3-phosphoglycerate dehydrogenase ELISA KIT ,Bovine 2-oxoglutarate reductase ELISA KIT ,Bovine 3-phosphoglycerate dehydrogenase ELISA KIT ,Bovine epididymis secretory protein Li 113 ELISA KIT ,Bovine malate dehydrogenase ELISA KIT
Intended use
Bovine PHGDH ELISA kit is for the in vitro quantitative determination of Bovine PHGDH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Bovine PHGDH ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PHGDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PHGDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PHGDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine PHGDH ELISA kit Range 1.56-100ng/ml
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