Bovine DDB1- and CUL4- associated factor 4, DCAF4 ELISA KIT

Product Name:Bovine DDB1- and CUL4- associated factor 4, DCAF4 ELISA KIT
Packing:96T

Catalog No.:ELI-25850b

Gene Name:Bovine DDB1- and CUL4-associated factor 4, DCAF4

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine DDB1- and CUL4-associated factor 4, DCAF4

Alternative Name:DCAF4,Bovine DDB1- and CUL4-associated factor 4, DCAF4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine DDB1- and CUL4- associated factor 4, DCAF4 ELISA KIT allows for the in vitro quantitative determination of Bovine DDB1- and CUL4-associated factor 4, DCAF4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine DDB1- and CUL4- associated factor 4, DCAF4 ELISA KIT has been pre-coated with an Bovine DDB1- and CUL4-associated factor 4, DCAF4 antibody specific to Bovine DDB1- and CUL4-associated factor 4, DCAF4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine DDB1- and CUL4-associated factor 4, DCAF4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine DDB1- and CUL4-associated factor 4, DCAF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine DDB1- and CUL4-associated factor 4, DCAF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!