Bovine Diacylglycerol O- acyltransferase 2, Dgat2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DGAT2,ARAT; GS1999FULL; acyl-CoA retinol O-fatty-acyltransferase; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; diglyceride acyltransferase 2; retinol O-fatty-acyltransferase; diacylglycerol O-acyltransferase 2
Search name
Bovine DGAT2 ELISA KIT ,Bovine ARAT ELISA KIT ,Bovine GS1999FULL ELISA KIT ,Bovine acyl-CoA retinol O-fatty-acyltransferase ELISA KIT ,Bovine diacylglycerol O-acyltransferase homolog 2 ELISA KIT ,Bovine diacylglycerol O-acyltransferase-like protein 2 ELISA KIT ,Bovine diglyceride acyltransferase 2 ELISA KIT ,Bovine retinol O-fatty-acyltransferase ELISA KIT ,Bovine diacylglycerol O-acyltransferase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Diacylglycerol O- acyltransferase 2, Dgat2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dgat2, During the reaction, Dgat2 in the sample or standard competes with a fixed amount of biotin-labeled Dgat2 for sites on a pre-coated Monoclonal antibody specific to Dgat2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dgat2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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