Bovine Elongation factor 2, EEF2 ELISA KIT

Starting at: $796.00

  • Model: ELI-26079b
  • 20 Units in Stock
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Bovine Elongation factor 2, EEF2 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Elongation factor 2,EF-2, EEF2,eukaryotic translation elongation factor 2; elongation factor 2; EEF-2; EF-2; EF2; polypeptidyl-tRNA translocase

 

Search name

Bovine Elongation factor 2 ELISA KIT ,Bovine EF-2 ELISA KIT ,Bovine  EEF2 ELISA KIT ,Bovine eukaryotic translation elongation factor 2 ELISA KIT ,Bovine elongation factor 2 ELISA KIT ,Bovine EEF-2 ELISA KIT ,Bovine EF-2 ELISA KIT ,Bovine EF2 ELISA KIT ,Bovine polypeptidyl-tRNA translocase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine elongation factor 2,EF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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