Bovine Enteropeptidase, TMPRSS15 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Bovine TMPRSS15 ELISA KIT ,Bovine ENTK ELISA KIT ,Bovine PRSS7 ELISA KIT ,Bovine enterokinase ELISA KIT ,Bovine enteropeptidase ELISA KIT ,Bovine protease serine 7 enterokinase ELISA KIT ,Bovine serine protease 7 ELISA KIT ,Bovine transmembrane protease serine 15 ELISA KIT

Intended use

Bovine TMPRSS15 ELISA KIT allows for the in vitro quantitative determination of bovine Enteropeptidase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Bovine TMPRSS15 ELISA KIT has been pre-coated with an antibody specific to Enteropeptidase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Enteropeptidase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Enteropeptidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Enteropeptidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bovine TMPRSS15 ELISA KIT Range 0.312-20.0 ng/ml

Bovine TMPRSS15 ELISA KIT PDF Manual download

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