Bovine Fibrinogen-like protein 1, FGL1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

FGL1,HFREP1; HP-041; LFIRE-1; LFIRE1; MFIRE-1, fibrinogen-like protein 1; fibrinogen-related protein 1, hepassocin; hepatocellular carcinoma-related sequence; hepatocyte-derived fibrinogen-related protein 1; liver fibrinogen-related protein 1; fibrinogen-like 1

Search name

Bovine FGL1 ELISA KIT ,Bovine HFREP1 ELISA KIT ,Bovine HP-041 ELISA KIT ,Bovine LFIRE-1 ELISA KIT ,Bovine LFIRE1 ELISA KIT ,Bovine MFIRE-1 ELISA KIT ,Bovine  fibrinogen-like protein 1 ELISA KIT ,Bovine fibrinogen-related protein 1 ELISA KIT ,Bovine  hepassocin ELISA KIT ,Bovine hepatocellular carcinoma-related sequence ELISA KIT ,Bovine hepatocyte-derived fibrinogen-related protein 1 ELISA KIT ,Bovine liver fibrinogen-related protein 1 ELISA KIT ,Bovine fibrinogen-like 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine Fibrinogen-like protein 1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen-like protein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forFibrinogen-like protein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Fibrinogen-like protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Fibrinogen-like protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.