Bovine Fibroblast growth factor 18, FGF18 ELISA KIT

Product Name:Bovine Fibroblast growth factor 18, FGF18 ELISA KIT
Packing:96T

Catalog No.:ELI-30815b

Gene Name:Bovine Fibroblast growth factor 18, FGF18

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine Fibroblast growth factor 18, FGF18

Alternative Name:FGF18,Bovine Fibroblast growth factor 18, FGF18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Fibroblast growth factor 18, FGF18 ELISA KIT allows for the in vitro quantitative determination of Bovine Fibroblast growth factor 18, FGF18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Fibroblast growth factor 18, FGF18 ELISA KIT has been pre-coated with an Bovine Fibroblast growth factor 18, FGF18 antibody specific to Bovine Fibroblast growth factor 18, FGF18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Fibroblast growth factor 18, FGF18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Fibroblast growth factor 18, FGF18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Fibroblast growth factor 18, FGF18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!