Bovine Fibulin 5, FBLN5 ELISA Kit

Bovine Fibulin 5, FBLN5 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Fibulin-5,FIBL-5,Developmental arteries and neural crest EGF-like protein,Dance, FBLN5,FIBL-5; ADCL2; ARCL1A; ARMD3; DANCE; EVEC; UP50; developmental arteries and neural crest EGF-like protein; fibulin-5; urine p50 protein; fibulin 5, Urine p50 protein(UP50),;

 

Search name

Bovine Fibulin-5 ELISA KIT ,Bovine FIBL-5 ELISA KIT ,Bovine Developmental arteries and neural crest EGF-like protein ELISA KIT ,Bovine Dance ELISA KIT ,Bovine  FBLN5 ELISA KIT ,Bovine FIBL-5 ELISA KIT ,Bovine ADCL2 ELISA KIT ,Bovine ARCL1A ELISA KIT ,Bovine ARMD3 ELISA KIT ,Bovine DANCE ELISA KIT ,Bovine EVEC ELISA KIT ,Bovine UP50 ELISA KIT ,Bovine developmental arteries and neural crest EGF-like protein ELISA KIT ,Bovine fibulin-5 ELISA KIT ,Bovine urine p50 protein ELISA KIT ,Bovine fibulin 5 ELISA KIT,Bovine Urine p50 protein ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine fibulin-5,FIBL-5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FIBL-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FIBL-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FIBL-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FIBL-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.