Bovine G- protein coupled bile acid receptor 1, GPBAR1 ELISA KIT

Product Name:Bovine G- protein coupled bile acid receptor 1, GPBAR1 ELISA KIT
Packing:96T

Catalog No.:ELI-48034b

Gene Name:Bovine G-protein coupled bile acid receptor 1, GPBAR1

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine G-protein coupled bile acid receptor 1, GPBAR1

Alternative Name:GPBAR1,Bovine G-protein coupled bile acid receptor 1, GPBAR1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine G- protein coupled bile acid receptor 1, GPBAR1 ELISA KIT allows for the in vitro quantitative determination of Bovine G-protein coupled bile acid receptor 1, GPBAR1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine G- protein coupled bile acid receptor 1, GPBAR1 ELISA KIT has been pre-coated with an Bovine G-protein coupled bile acid receptor 1, GPBAR1 antibody specific to Bovine G-protein coupled bile acid receptor 1, GPBAR1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine G-protein coupled bile acid receptor 1, GPBAR1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine G-protein coupled bile acid receptor 1, GPBAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine G-protein coupled bile acid receptor 1, GPBAR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!