Bovine FABP6 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name FABP6
Protein Name Gastrotropin
Alternative Name
Bovine FABP6 ELISA KIT ,Bovine I-15P ELISA KIT ,Bovine I-BABP ELISA KIT ,Bovine I-BALB ELISA KIT ,Bovine I-BAP ELISA KIT ,Bovine ILBP ELISA KIT ,Bovine ILBP3 ELISA KIT ,Bovine ILLBP ELISA KIT ,Bovine gastrotropin ELISA KIT ,Bovine fatty acid binding protein 6 ELISA KIT ,Bovine ileal ELISA KIT ,Bovine ileal bile acid binding protein ELISA KIT ,Bovine ileal lipid-binding protein ELISA KIT ,Bovine illeal lipid-binding protein ELISA KIT ,Bovine intestinal 15 kDa protein ELISA KIT ,Bovine fatty acid binding protein 6 ELISA KIT
Intended use
Bovine FABP6 ELISA KIT allows for the in vitro quantitative determination of FABP6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Bovine FABP6 ELISA KIT has been pre-coated with an FABP6 antibody specific to FABP6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FABP6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FABP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FABP6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20