Bovine SLC35C1 ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name SLC35C1
Protein Name GDP- fucose transporter 1/ solute carrier family 35 member C1
Alternative Name
Bovine SLC35C1 ELISA KIT ,Bovine CDG2C ELISA KIT ,Bovine FUCT1 ELISA KIT ,Bovine GDP-fucose transporter 1 ELISA KIT ,Bovine solute carrier family 35 (GDP-fucose transporter) ELISA KIT ,Bovine member C1 ELISA KIT ,Bovine solute carrier family 35 member C1 ELISA KIT
Bovine SLC35C1 ELISA Kit Range 1.56-100ng/mL
Intended use
Bovine SLC35C1 ELISA KIT allows for the in vitro quantitative determination of SLC35C1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Bovine SLC35C1 ELISA KIT has been pre-coated with an SLC35C1 antibody specific to SLC35C1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SLC35C1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SLC35C1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SLC35C1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20