Bovine Glutathione peroxidase 1, GPX1 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Glutathione peroxidase 1(GPx-1,GSHPx-1),Cellular glutathione peroxidase, Gpx1,Gpx; GPx-1; AI195024; AL033363; CGPx; GSHPx-1;
cellular glutathione peroxidase; Glutathione peroxidase 1

Search name
Bovine Glutathione peroxidase 1 ELISA Kit, Bovine GPx-1 ELISA Kit, Bovine GSHPx-1 ELISA Kit, Bovine GPx1 ELISA Kit, Bovine
Cellular glutathione peroxidase ELISA Kit, Bovine GPx ELISA Kit, Bovine AI195024 ELISA Kit, Bovine AL033363 ELISA Kit,
Bovine CGPx ELISA Kitd

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine glutathione peroxidase 1,GPx-1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPx-1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPx-1 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain GPx-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GPx-1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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