Bovine Heat shock 70 kDa protein 1B, HSPA1B ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Heat shock 70 kDa protein 1B,Heat shock 70 kDa protein 2(HSP70.2),; HSP70-1B; HSP70-2; HSPA1B; HSP70-2; HSP70.2; heat shock
70 kDa protein 2; heat shock 70 kDa protein 1B;

Search name
Bovine Heat shock 70 kDa protein 1B ELISA Kit, Bovine HSPA1B ELISA Kit, Bovine Heat shock 70 kDa protein 2 ELISA Kit,
Bovine HSP70.2 ELISA Kit, Bovine HSP70-1B ELISA Kit, Bovine HSP70-2 ELISA Kit, Bovine H HSP70-2 ELISA Kit, Bovine heat
shock 70 kDa protein 2 ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Heat shock 70 kDa protein 1B concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat shock 70 kDa protein 1B. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Heat
shock 70 kDa protein 1B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a
TMB substrate solution is added to each well. Only those wells that contain Heat shock 70 kDa protein 1B, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heat shock 70
kDa protein 1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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