Bovine Heparanase, HPSE ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HPA; HPA1; HPR1; HPSE1; HSE1; endo-glucoronidase; heparanase-1,HPSE,HEP;HPA;HPA1;HPR1;HPSE1;HSE1

Search name

Bovine HPA ELISA KIT ,Bovine HPA1 ELISA KIT ,Bovine HPR1 ELISA KIT ,Bovine HPSE1 ELISA KIT ,Bovine HSE1 ELISA KIT ,Bovine endo-glucoronidase ELISA KIT ,Bovine heparanase-1 ELISA KIT ,Bovine HPSE ELISA KIT ,Bovine HEP ELISA KIT ,Bovine HPA ELISA KIT ,Bovine HPA1 ELISA KIT ,Bovine HPR1 ELISA KIT ,Bovine HPSE1 ELISA KIT ,Bovine HSE1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Heparanase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparanase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Heparanase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heparanase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Heparanase in the samples is then determined by comparing the O.D. of the samples to the standard curve.