Bovine Hepcidin, HEPC ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Hepcidin,Liver-expressed antimicrobial peptide 1,LEAP-1,Putative liver tumor regressor,PLTR, HAMP,hepcidin antimicrobial peptide;
hepcidin; HEPC; HFE2B; LEAP1; PLTR; liver-expressed antimicrobial peptide 1

Search name
Bovine Hepcidin ELISA KIT,Bovine Liver-expressed antimicrobial peptide 1 ELISA KIT,Bovine LEAP-1 ELISA KIT,Bovine Putative
liver tumor regressor ELISA KIT,Bovine PLTR ELISA KIT,Bovine HAMP ELISA KIT,Bovine hepcidin antimicrobial peptide ELISA
KIT,Bovine hepcidin ELISA KIT,Bovine HEPC ELISA KIT,Bovine HFE2B ELISA KIT,Bovine LEAP1 ELISA KIT,Bovine PLTR ELISA
KIT,Bovine liver-expressed antimicrobial peptide 1 ELISA KIT,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Hepcidin concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hepcidin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Hepcidin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hepcidin in the samples is then determined by comparing
the O.D. of the samples to the standard curve

PDF manual download