Bovine Insulin like growth factor I, IGF1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Insulin-like growth factor I,IGF-I,Somatomedin, IGF1,IGF-1,IGF-I; IGF1A; IGFI; IGF-IA; IGF-IB; MGF; insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C; insulin-like growth factor 1

Search name

Bovine Insulin-like growth factor I ELISA KIT ,Bovine IGF-I ELISA KIT ,Bovine Somatomedin ELISA KIT ,Bovine  IGF1 ELISA KIT ,Bovine IGF-1 ELISA KIT ,Bovine IGF-I ELISA KIT ,Bovine IGF1A ELISA KIT ,Bovine IGFI ELISA KIT ,Bovine IGF-IA ELISA KIT ,Bovine IGF-IB ELISA KIT ,Bovine MGF ELISA KIT ,Bovine insulin-like growth factor 1 ELISA KIT ,Bovine insulin-like growth factor I ELISA KIT ,Bovine insulin-like growth factor IA ELISA KIT ,Bovine insulin-like growth factor IB ELISA KIT ,Bovine mechano growth factor ELISA KIT ,Bovine somatomedin-C ELISA KIT ,Bovine insulin-like growth factor 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine insulin-like growth factor i,IGF-I concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-I. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IGF-I and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IGF-I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IGF-I in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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