Bovine Interleukin-10, IL-10 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-10(IL-10),Cytokine synthesis inhibitory factor(CSIF),

Search name
Bovine Interleukin-10 ELISA Kit, Bovine IL-10 ELISA Kit, Bovine IL10 ELISA Kit,Interleukin-10 ELISA Kit,IL-10 ELISA Kit,IL10
ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine interleukin-10,IL-10 concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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