Bovine Islet amyloid polypeptide, IAPP ELISA KIT
Product Name:Bovine Islet amyloid polypeptide, IAPP ELISA KIT
Packing:96T
Catalog No.:ELI-02740b
Gene Name:Bovine Islet amyloid polypeptide, IAPP
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Islet amyloid polypeptide, IAPP
Alternative Name:IAPP,Bovine Islet amyloid polypeptide, IAPP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Islet amyloid polypeptide, IAPP ELISA KIT allows for the in vitro quantitative determination of Bovine Islet amyloid polypeptide, IAPP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Islet amyloid polypeptide, IAPP ELISA KIT has been pre-coated with an Bovine Islet amyloid polypeptide, IAPP antibody specific to Bovine Islet amyloid polypeptide, IAPP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Islet amyloid polypeptide, IAPP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Islet amyloid polypeptide, IAPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Islet amyloid polypeptide, IAPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!